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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/09/2017 |
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Data da última atualização: |
31/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
INBRAHIM, A. B.; MONTEIRO, T. R.; CABRAL, G. B.; ARAGAO, F. J. L. |
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Afiliação: |
Abdulrazak B. Ibrahim, UnB; Tatiane R. Monteiro, UnB; GLAUCIA BARBOSA CABRAL, Cenargen; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
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Título: |
RNAi-mediated resistance to whitefly (Bemisia tabaci) in genetically engineered lettuce (Lactuca sativa). |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Transgenic Research, v. 26, p. 613-624, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
RNA interference (RNAi)-based transgenic technologies have evolved as potent biochemical tools for silencing specific genes of plant pathogens and pests. The approach has been demonstrated to be useful in silencing genes in insect species. Here, we report on the successful construction of RNAi-based plasmid containing an interfering cassette designed to generate dsRNAs that target a novel v-ATPase transcript in whitefly (Bemisia tabaci), an important agricultural pest in tropical and sub-tropical regions. The presence of the transgene was confirmed in T0 and T1 generations of transgenic lettuce lines, segregating in a Mendelian fashion. Seven lines were infested with whiteflies and monitored over a period of 32 days. Analysis of mortality showed that within five days of feeding, insects on transgenic plants showed a mortality rate of 83.8?98.1%. In addition, a reduced number of eggs (95 fold less) was observed in flies feeding on transgenic lettuce plants than insects on control lines. Quantitative reverse transcription PCR showed decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. This technology is a foundation for the production of whitefly-resistant commercial crops, improving agricultural sustainability and food security, reducing the use of more environmentally aggressive methods of pest control. |
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Palavras-Chave: |
ATPase; Horticultural crop; Insect resistance; SiRNA. |
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Thesaurus Nal: |
genetic engineering. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02014naa a2200217 a 4500
001 2076425
005 2023-03-31
008 2017 bl uuuu u00u1 u #d
100 1 $aINBRAHIM, A. B.
245 $aRNAi-mediated resistance to whitefly (Bemisia tabaci) in genetically engineered lettuce (Lactuca sativa).$h[electronic resource]
260 $c2017
520 $aRNA interference (RNAi)-based transgenic technologies have evolved as potent biochemical tools for silencing specific genes of plant pathogens and pests. The approach has been demonstrated to be useful in silencing genes in insect species. Here, we report on the successful construction of RNAi-based plasmid containing an interfering cassette designed to generate dsRNAs that target a novel v-ATPase transcript in whitefly (Bemisia tabaci), an important agricultural pest in tropical and sub-tropical regions. The presence of the transgene was confirmed in T0 and T1 generations of transgenic lettuce lines, segregating in a Mendelian fashion. Seven lines were infested with whiteflies and monitored over a period of 32 days. Analysis of mortality showed that within five days of feeding, insects on transgenic plants showed a mortality rate of 83.8?98.1%. In addition, a reduced number of eggs (95 fold less) was observed in flies feeding on transgenic lettuce plants than insects on control lines. Quantitative reverse transcription PCR showed decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. This technology is a foundation for the production of whitefly-resistant commercial crops, improving agricultural sustainability and food security, reducing the use of more environmentally aggressive methods of pest control.
650 $agenetic engineering
653 $aATPase
653 $aHorticultural crop
653 $aInsect resistance
653 $aSiRNA
700 1 $aMONTEIRO, T. R.
700 1 $aCABRAL, G. B.
700 1 $aARAGAO, F. J. L.
773 $tTransgenic Research$gv. 26, p. 613-624, 2017.
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| 1. |  | 1100056, CANADIAN PLANT DISEASE SURVEY, Research Branch Agriculture Canada, Ottawa-Canada
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Agropecuária Oeste; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Trigo; Embrapa Uva e Vinho. |   |
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